It has been a while since I posted here…but we need data. Human, primary cells were our biggest sellers in 2016. Most can be passaged 6+ times so users have been able to build healthy stocks. In searching for new business, I am learning that many labs need more data in order to determine if our cells will work for them. This has resulted in our Desperately Seeking Data. Just email us any data generated using our cells and we will email back a 25 USD Starbuck’s Gift Card.
Here’s an example of customer data provided as part of this campaign.
Neuromics’ Neurons in Culture-Courtesy of Dr. Mahendran Subramanian, Keele University
Figure. Gene delivery by oscillating nanomagnetic gene transfection in primary cortical neurons. Images of pmaxGFP plasmid expressed in primary neurons using fluorescence microscopy and its corresponding Hoechst 33,342 stained counterpart of transfected DIV 1 (A,C) and DIV 5 (B,D) mature neurons were taken 48 h post transfection
Motor Neurons and MEA
Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem’s Maestro MEA.
Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays
A common Neuromics’ theme is harnessing the power of cellsTM. The raw cost of the cells are often the biggest consideration. We encourage our customers to focus on true costs. These include the # of cells (how many times can they be passaged?), culture viability (how long do the cells live) and bioactivity (how closely do cultures mimic in vivo behavior?). I would like to present a presentation and publication confirming our competitive advantage when analyzing true costs.
Setting a higher bar for Neuron-Glial Based Assays!
Dr. Randen Patterson and his team at UC Davis have developed new culturing techniques using our e18 Rat Primary Hippocampal Neurons. They have developed a protocol that allows for culturing of E18 hippocampal neurons at high densities for more than 120 days. These cultured hippocampal neurons are (i) well differentiated with high numbers of synapses, (ii) anchored securely to their substrate, (iii) have high levels of functional connectivity, and (iv) form dense multi-layered cellular networks. We propose that our culture methodology is likely to be effective for multiple neuronal subtypes–particularly those that can be grown in Neurobasal/B27 media. This methodology presents new avenues for long-term functional studies in neurons. This is good news indeed: Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. PLoS ONE 8(4): e58996. doi:10.1371/journal.pone.0058996.
We will continue to raise the bar. Better cultures=lower costs and better outcomes!
Coming soon…Now for something completely different…Synaptic Transmission Research.
I am pleased to be featuring Dr. Evanna Gleason. I selected her because She and her team’s research is a basic component of most areas Neuroscience Research including pain, neurodegeneration, vision, TBI, SCI, drug addiction, neuro-disorders and more…This base component is how neurons communicate with other cells at synapses.
She focuses on synaptic transmission in the vertebrate retina. Retinal neurons have distinctive anatomical and physiological properties that suggest they employ unique synaptic mechanisms. The long term objective her research is to understand how retinal synapses are specialized to transmit visual information.