Network vs Individual Bursting Neurons

Motor Neurons and MEA
Dysregulated bursting is at the root of many motor neuron/neuromuscular junction disease. ArunA Biomedical teaming with Axion Biosystems have generated relevant bursting data from our Mouse Motor Neurons cultured on Axion-Bioystem’s Maestro MEA.

Figure: Mouse Motor Neuron Network Modulation by Bicuculline-ckeck out the entire presentation to learn more: GFP+ Motor Neurons: Development and in-vitro Functional Assessment on Microelectrode Arrays

Protocol User’s Guide for Culturing Motor Neuron on MEA(pdf – 679Kb)

Name Catalog # Type Species Applications Size Price
Motor Neurons-GFP+ Quick Start Kit mMN7205.QS Primary Neurons M Cell Assays 750,000 $349
Motor Neurons-GFP+ HTS Kit mMN7205-HTS Primary Neurons M Cell Assays 4 X 750,000 $989
GDNF (Human, Mouse) PR27022-2 Protein H; M 2 ug 10 ug $108 $205
AB2™ Basal Neural Medium AB27011.3 Cell Growth Media H; M Cell Assays 500 ml $69

We will continue providing you content we believe important. Should you have questions, do not hesitate to contact us. Thank you and we stand ready to serve you and your team.

Pete Shuster-CEO and Owner, Neuromics, 612-801-1007, pshuster@neuromics.com

TCE and Parkinson’s

This post introduces a new twist to “News Behind the Neuroscience News”. This report was graciously submitted by Ms Pamela Brown of associatesdegree.com. I welcome her initiative in submitting this. On a side note, when I did factory work in the 1970s, trichloroethylene was a universal solvent used to clean parts. Wow. We will add more to the story as Researchers continue their studies. Thank you Pamela!

Of Chemicals and Parkinson’s Disease

Two recent studies have linked the chemical TCE, an industrial solvent, to the increased risk of Parkinson’s disease.  The first study, led by the University of Kentucky’s Don M. Gash and John T. Slevin, established a clear link between trichloroethylene and parkinsonism , which is a group of nervous disorders closely associated with Parkinson’s.

 The study investigated a group of people who had been occupationally exposed to TCE for over twenty-five years. Gash and Slevin found that of the 134 participants interviewed, 14–the group that worked closest to the TCE vat cleaning industrial parts–showed strong signs of Parkinsonism. 13 other patients who had worked further from the TCE source also showed signs of the disorder, although in milder form. The University of Kentucky study extended its investigation by exposing rats to TCE. The rats’ mitochondrial function was substantially inhibited and their dopamine-producing cells were severely damaged.

 A more recent study , revealed in January, established an even stronger link between TCE and Parkinson’s. Dr. Samuel Goldman, a researcher at the Parkinson’s Institute in Sunnyvale, California, examined 99 sets of twins in which one twin had Parkinson’s and the other didn’t. Goldman and his team gathered job histories from the group of twins and had an industrial hygienist evaluate the twins’ level of chemical exposure. The study found that those exposed to TCE had an almost six-fold increased risk of developing Parkinson’s. Occupations that often involve exposure to TCE include machinists, laundry cleaners, and electricians.

Even though the most recent findings are substantial, the idea that chemicals may be associated with increased risk of developing Parkinson’s is not very new. Earlier studies have suggested that certain pesticides and herbicides may increase risk as well.

Not all chemicals are bad news for Parkinson’s patients. A brain chemical, identified only about twenty years ago and named after the video game character, Sonic Hedgehog , has been shown to decrease the risk of developing Parkinson’s, meaning that increasing the chemical may be a viable treatment for the disease. Another study has suggested that urate, a naturally occurring chemical in the blood, may slow the progression of Parkinson’s, although the chemical has been proven to cause gout.

By-line:

This guest post is contributed by Pamelia Brown, who writes on the topics of associate degree .  She welcomes your comments at her email Id: pamelia.brown@gmail.com

Dr. Steve Stice Podcast

Inteview with Steve Stice.

Podcast by ClearCast

This Podcast gives is a good primer on Embryonic Stem Cells (ESCs)…where they come from, how they are used and the promise they have for helping researchers cure human diseases.

The listener will gain insight how ECSs when manipulated into mature neuronal cell lines can accelerate the pace of neurological research for scientists working on treatments for spinal cord injuries and neurological diseases such as Parkinson’s, Huntington’s, Alzheimer’s, ALS and possibly even depression.

On Deck-Dr. Steve Stice

We read about the promise of stem cells in the news every day. They could prove to be ”magic bullets” for curing diseases like Alzheimer’s. Parkinson’s, MS and others. Stem Cell Research is also surrounded with controversy as currently cells are often harvested from human embryos and fetuses.I believe top researchers will prove to be the voice of reason in the human stem cell debate as they are the ones best positioned to know the risks, limitations and potential.  

For our August Profile, I am honored to be featuring Dr. Steve Stice. I have had the pleasure of working with Dr. Stice both in his role as Professor and Director of the Regenerative Bioscience Center and Research Alliance Eminent Scholar endowed Chair at the University of Georgia and as Founder and Chief Scientific Officer at Aruna Biomedical.

He has over 16 years of research and development experience in biotechnology and is a co-founder of five biotechnology companies.  He was named one of the 100 Most Influential Georgians by Georgia Trend magazine.  He produced the first cloned rabbit in 1987 and the first cloned transgenic calves in 1998 (George and Charlie).  In 1997 his group produced the first genetically modified embryonic stem cell derived pigs and cattle.  This research led to publications in Science and Nature journals, national news coverage (CBS, NBC, ABC and CNN) and the first US patents on cloning animals and cattle embryonic stem cells.  In 2001, Dr. Stice announced the first cloned animal (calf) from an animal that was dead for 48 hours.  In 2005, his stem cell group published the first work on deriving motor neurons from stem cells.  Motor neurons are damaged lost during the progression of several diseases such as ALS and spinal muscular atrophy.  Throughout his career he has published and lectured on cloning and stem cell technologies.  Prior to joining the University of Georgia, Dr. Stice was a co-founder and Chief Scientific Officer at Advanced Cell Technology, a company developing cloning and stem cell technology.

Here is What is  Currently Hot in the Stice Lab:
New neural stem cells technology developed in my lab was transferred to a commercial entity, Aruna biomedical. This is the first commercialized product derived from human embryonic stem cell using federally approved stem cell lines.

  • We have produced neurons that have neural functions
  • We are working with the Navy to use our neural cells as biosensors for environmental toxins 
  • We have vascular stem cells that have characteristics that may make them suitable for  transplantation
  • We collaborate with a new company call Aruna BioMedical  that will stem cells for neural research and drug discovery
  • Developed a method to test new compounds for Alzheimer’s disease using our neural stem cell
  • We are one of five NIH stem cell training centers and have taught Scientists from Georgia to Bombay India new stem cell techniques
  • In Georgia, we produced over 50 cloned calves and 100 cloned pigs.
  • We were also the first to produce a clone from an animal that had been dead for 48 hours. This opens new opportunities in agriculture and preserving endangered species.